Services Provided by the Genome Editing Core

The GECF offers the following services (more to follow):

CRISPR/CAS9 -Mediated Gene Editing in Mouse

  • gene knock-out
  • gene replacement (amino acid changes)
  • epitope tagging, including fluorescent protein tagging
  • conditional models

DNA Microinjection for transgenic generation

  • Transgene DNA Purification available as an additional service

BAC DNA Microinjection for BAC transgenesis

ES Cell Line Injection

  • ES Cell preparation and Karyotyping available as additional services

CRISPR Target Validation (for Gene Editing in Mouse)

Mouse Cryopreservation:

  • Sperm Cryopreservation
  • Embryo Cryopreservation

In Vitro Fertilization

  • Frozen/Fresh sperm for line import, recovery or rapid expansion

Mouse Line Rederivation

  • Live Mice as Source
  • Frozen Embryos as Source

Genotyping

  • The GECF offers genotyping of lines generated as an additional service
  • The GECF will design and validate genotyping assays as needed

 

Fees for GECF services are available upon request, please email gecf-ru@rutgers.edu